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fluorescence microscope nikon c2 confocal microscope  (Nikon)


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    Nikon fluorescence microscope nikon c2 confocal microscope
    Fluorescence Microscope Nikon C2 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+confocal+microscope+nikon+c2/pmc10826405-60-5-7
    Average 90 stars, based on 1 article reviews
    fluorescence microscope nikon c2 confocal microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Regulation of microglia polarization via mannose receptor-mediated delivery of siRNA by ligand-functionalized DoGo LNP
    Article Snippet: For visualization, the secondary antibodies, anti-rabbit Alexa-594 (1 : 1000; Cell Signaling Technology, MA, USA), or goat anti-rabbit IgG-FITC (1 : 1000; Abcam, Cambridge, MA) were used along with the nuclear marker DAPI (1 : 1000; Thermo Scientific, MA, USA). .. Images were obtained by a Nikon fluorescence confocal microscope (Nikon, C2 + ). .. After BV2 cells were treated with siTLR4/M3 or siCtrl/M3 complexes followed by OGD (see section of siRNA transfection efficiency), the cell culture conditioned media were added into precultured neuron cells (N2a) and incubated for 24 h, and then, apoptotic cells and the cell viability were measured using a calcein AM/PI double staining kit (Yeasen, Shanghai, China) and the CellTiter 96 Aqueous One Solution Cell Proliferation assay (Promega, WI, USA), respectively, following the manufacturer's instructions.

    Article Title: Targeted Nanodrugs to Destroy the Tumor Extracellular Matrix Barrier for Improving Drug Delivery and Cancer Therapeutic Efficacy.
    Article Snippet: One of the main reasons why most cancer patients do not respond well to chemotherapy is that drugs cannot accumulate in tumors at an optimal dose, eventually resulting in failure to prevent cancer cell growth.. To improve drug delivery efficiency, we engineered a highly efficient tumor-targeted and stroma-breaking nanocarrier by the modification of iron oxide nanoparticles (IONPs) with a tumor-targeting peptide c(RGDyK) and a hyaluronidase (HAase) on the surface.. The yielding nanocomplex, c(RGDyK)-HAase-IONP, targeted the tumor by binding integrin αvβ3 and went deeply into the tumors by the degradation of hyaluronic acid (HA), which was highly expressed in the tumor extracellular matrix (ECM).

    Microscopy:

    Article Title: Regulation of microglia polarization via mannose receptor-mediated delivery of siRNA by ligand-functionalized DoGo LNP
    Article Snippet: For visualization, the secondary antibodies, anti-rabbit Alexa-594 (1 : 1000; Cell Signaling Technology, MA, USA), or goat anti-rabbit IgG-FITC (1 : 1000; Abcam, Cambridge, MA) were used along with the nuclear marker DAPI (1 : 1000; Thermo Scientific, MA, USA). .. Images were obtained by a Nikon fluorescence confocal microscope (Nikon, C2 + ). .. After BV2 cells were treated with siTLR4/M3 or siCtrl/M3 complexes followed by OGD (see section of siRNA transfection efficiency), the cell culture conditioned media were added into precultured neuron cells (N2a) and incubated for 24 h, and then, apoptotic cells and the cell viability were measured using a calcein AM/PI double staining kit (Yeasen, Shanghai, China) and the CellTiter 96 Aqueous One Solution Cell Proliferation assay (Promega, WI, USA), respectively, following the manufacturer's instructions.

    Article Title: Targeted Nanodrugs to Destroy the Tumor Extracellular Matrix Barrier for Improving Drug Delivery and Cancer Therapeutic Efficacy.
    Article Snippet: One of the main reasons why most cancer patients do not respond well to chemotherapy is that drugs cannot accumulate in tumors at an optimal dose, eventually resulting in failure to prevent cancer cell growth.. To improve drug delivery efficiency, we engineered a highly efficient tumor-targeted and stroma-breaking nanocarrier by the modification of iron oxide nanoparticles (IONPs) with a tumor-targeting peptide c(RGDyK) and a hyaluronidase (HAase) on the surface.. The yielding nanocomplex, c(RGDyK)-HAase-IONP, targeted the tumor by binding integrin αvβ3 and went deeply into the tumors by the degradation of hyaluronic acid (HA), which was highly expressed in the tumor extracellular matrix (ECM).

    Immunofluorescence:

    Article Title: Targeted Nanodrugs to Destroy the Tumor Extracellular Matrix Barrier for Improving Drug Delivery and Cancer Therapeutic Efficacy.
    Article Snippet: One of the main reasons why most cancer patients do not respond well to chemotherapy is that drugs cannot accumulate in tumors at an optimal dose, eventually resulting in failure to prevent cancer cell growth.. To improve drug delivery efficiency, we engineered a highly efficient tumor-targeted and stroma-breaking nanocarrier by the modification of iron oxide nanoparticles (IONPs) with a tumor-targeting peptide c(RGDyK) and a hyaluronidase (HAase) on the surface.. The yielding nanocomplex, c(RGDyK)-HAase-IONP, targeted the tumor by binding integrin αvβ3 and went deeply into the tumors by the degradation of hyaluronic acid (HA), which was highly expressed in the tumor extracellular matrix (ECM).

    Staining:

    Article Title: Targeted Nanodrugs to Destroy the Tumor Extracellular Matrix Barrier for Improving Drug Delivery and Cancer Therapeutic Efficacy.
    Article Snippet: One of the main reasons why most cancer patients do not respond well to chemotherapy is that drugs cannot accumulate in tumors at an optimal dose, eventually resulting in failure to prevent cancer cell growth.. To improve drug delivery efficiency, we engineered a highly efficient tumor-targeted and stroma-breaking nanocarrier by the modification of iron oxide nanoparticles (IONPs) with a tumor-targeting peptide c(RGDyK) and a hyaluronidase (HAase) on the surface.. The yielding nanocomplex, c(RGDyK)-HAase-IONP, targeted the tumor by binding integrin αvβ3 and went deeply into the tumors by the degradation of hyaluronic acid (HA), which was highly expressed in the tumor extracellular matrix (ECM).

    Labeling:

    Article Title: Targeted Nanodrugs to Destroy the Tumor Extracellular Matrix Barrier for Improving Drug Delivery and Cancer Therapeutic Efficacy.
    Article Snippet: One of the main reasons why most cancer patients do not respond well to chemotherapy is that drugs cannot accumulate in tumors at an optimal dose, eventually resulting in failure to prevent cancer cell growth.. To improve drug delivery efficiency, we engineered a highly efficient tumor-targeted and stroma-breaking nanocarrier by the modification of iron oxide nanoparticles (IONPs) with a tumor-targeting peptide c(RGDyK) and a hyaluronidase (HAase) on the surface.. The yielding nanocomplex, c(RGDyK)-HAase-IONP, targeted the tumor by binding integrin αvβ3 and went deeply into the tumors by the degradation of hyaluronic acid (HA), which was highly expressed in the tumor extracellular matrix (ECM).



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    Reduced PRC2 complex in Tau-KO SH-SY5Y cells. (A) . Shown are matched protein amounts of parental (WT) or Tau-KO cell lysates analyzed by western blot (biological triplicates on a single gel) with EZH2, SUZ12 or GAPDH primary antibodies and anti-rabbit IgG IRDye 800CW secondary antibody. The EZH2 and SUZ12 signals were normalized on the respective GAPDH signals and reported as fold of WT; mean ± SD of 9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (B) . Determination of nuclear EZH2 or SUZ12 mean <t>fluorescent</t> intensity analyzed by immune fluorescence staining and laser confocal <t>microscopy</t> with EZH2 or SUZ12 antibodies revealed with an anti-rabbit AlexaFluor 488 antibody. Data obtained with a DAPI nuclear mask (ImageJ) are reported as fold of WT mean value of 292–475 nuclei from two (EZH2) or three (SUZ12) independent experiments, unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). (C) . Shown are RT-PCR determination of mRNA with specific primers for EZH2 or SUZ12. Normalization was performed on the geometric mean of the GAPDH and HPRT1 mRNA values and reported as fold of WT; mean ± SD of 12 biological replicates, unpaired Mann-Whitney test. (D) . Shown are matched protein amounts of cell lysates subjected to immune isolation (IP) with EZH2 or SUZ12 antibodies or matched amounts of control antibodies (ctrl IP). Samples were resolved on a single same gel and analyzed by western blot with EZH2 or SUZ12 antibodies and secondary anti-rabbit IgG IRDye 800CW antibody.
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    Image Search Results


    Reduced PRC2 complex in Tau-KO SH-SY5Y cells. (A) . Shown are matched protein amounts of parental (WT) or Tau-KO cell lysates analyzed by western blot (biological triplicates on a single gel) with EZH2, SUZ12 or GAPDH primary antibodies and anti-rabbit IgG IRDye 800CW secondary antibody. The EZH2 and SUZ12 signals were normalized on the respective GAPDH signals and reported as fold of WT; mean ± SD of 9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (B) . Determination of nuclear EZH2 or SUZ12 mean fluorescent intensity analyzed by immune fluorescence staining and laser confocal microscopy with EZH2 or SUZ12 antibodies revealed with an anti-rabbit AlexaFluor 488 antibody. Data obtained with a DAPI nuclear mask (ImageJ) are reported as fold of WT mean value of 292–475 nuclei from two (EZH2) or three (SUZ12) independent experiments, unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). (C) . Shown are RT-PCR determination of mRNA with specific primers for EZH2 or SUZ12. Normalization was performed on the geometric mean of the GAPDH and HPRT1 mRNA values and reported as fold of WT; mean ± SD of 12 biological replicates, unpaired Mann-Whitney test. (D) . Shown are matched protein amounts of cell lysates subjected to immune isolation (IP) with EZH2 or SUZ12 antibodies or matched amounts of control antibodies (ctrl IP). Samples were resolved on a single same gel and analyzed by western blot with EZH2 or SUZ12 antibodies and secondary anti-rabbit IgG IRDye 800CW antibody.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Tau protein modulates an epigenetic mechanism of cellular senescence in human SH-SY5Y neuroblastoma cells

    doi: 10.3389/fcell.2023.1232963

    Figure Lengend Snippet: Reduced PRC2 complex in Tau-KO SH-SY5Y cells. (A) . Shown are matched protein amounts of parental (WT) or Tau-KO cell lysates analyzed by western blot (biological triplicates on a single gel) with EZH2, SUZ12 or GAPDH primary antibodies and anti-rabbit IgG IRDye 800CW secondary antibody. The EZH2 and SUZ12 signals were normalized on the respective GAPDH signals and reported as fold of WT; mean ± SD of 9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (B) . Determination of nuclear EZH2 or SUZ12 mean fluorescent intensity analyzed by immune fluorescence staining and laser confocal microscopy with EZH2 or SUZ12 antibodies revealed with an anti-rabbit AlexaFluor 488 antibody. Data obtained with a DAPI nuclear mask (ImageJ) are reported as fold of WT mean value of 292–475 nuclei from two (EZH2) or three (SUZ12) independent experiments, unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). (C) . Shown are RT-PCR determination of mRNA with specific primers for EZH2 or SUZ12. Normalization was performed on the geometric mean of the GAPDH and HPRT1 mRNA values and reported as fold of WT; mean ± SD of 12 biological replicates, unpaired Mann-Whitney test. (D) . Shown are matched protein amounts of cell lysates subjected to immune isolation (IP) with EZH2 or SUZ12 antibodies or matched amounts of control antibodies (ctrl IP). Samples were resolved on a single same gel and analyzed by western blot with EZH2 or SUZ12 antibodies and secondary anti-rabbit IgG IRDye 800CW antibody.

    Article Snippet: Detection by fluorescent laser confocal microscopy (Nikon C2 microscope) was done with 2 μg/mL secondary antibodies anti-mouse IgG Alexa594, anti-rabbit IgG -Alexa 488 or anti-rabbit IgG-Alexa 647 (A-11032, A-11034, A21245, Thermo Fisher Scientific).

    Techniques: Western Blot, MANN-WHITNEY, Fluorescence, Staining, Confocal Microscopy, Reverse Transcription Polymerase Chain Reaction, Isolation, Control

    Reduced PRC2 activity in Tau-KO SH-SY5Y cells. (A) . Shown are matched protein amounts of parental (WT) or Tau-KO cell lysates analyzed by western blot (biological triplicates on a single gel) with H3K27me3, H3 or GAPDH primary antibodies and anti-rabbit IgG IRDye 800CW secondary antibody. The H3K27me3 and H3 signals were normalized for GAPDH and reported as fold of WT for the H3K27me3/H3 ratios; mean ± SD of 8-9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (B) . Nuclear H3K27me3 mean fluorescent intensity was determined by immune fluorescence staining and laser confocal microscopy. Shown are representative confocal microscopy images, scale bar 20 μm. Data obtained with a DAPI nuclear mask (ImageJ) are reported as fold of WT mean value of 695–716 nuclei from five independent experiments, unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). (C) . Shown are RT-PCR determination of mRNA with specific primers as indicated. Normalization was performed on the geometric mean of the GAPDH and HPRT1 mRNA values and reported as fold of WT; mean ± SD of 3–12 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Tau protein modulates an epigenetic mechanism of cellular senescence in human SH-SY5Y neuroblastoma cells

    doi: 10.3389/fcell.2023.1232963

    Figure Lengend Snippet: Reduced PRC2 activity in Tau-KO SH-SY5Y cells. (A) . Shown are matched protein amounts of parental (WT) or Tau-KO cell lysates analyzed by western blot (biological triplicates on a single gel) with H3K27me3, H3 or GAPDH primary antibodies and anti-rabbit IgG IRDye 800CW secondary antibody. The H3K27me3 and H3 signals were normalized for GAPDH and reported as fold of WT for the H3K27me3/H3 ratios; mean ± SD of 8-9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (B) . Nuclear H3K27me3 mean fluorescent intensity was determined by immune fluorescence staining and laser confocal microscopy. Shown are representative confocal microscopy images, scale bar 20 μm. Data obtained with a DAPI nuclear mask (ImageJ) are reported as fold of WT mean value of 695–716 nuclei from five independent experiments, unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). (C) . Shown are RT-PCR determination of mRNA with specific primers as indicated. Normalization was performed on the geometric mean of the GAPDH and HPRT1 mRNA values and reported as fold of WT; mean ± SD of 3–12 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218).

    Article Snippet: Detection by fluorescent laser confocal microscopy (Nikon C2 microscope) was done with 2 μg/mL secondary antibodies anti-mouse IgG Alexa594, anti-rabbit IgG -Alexa 488 or anti-rabbit IgG-Alexa 647 (A-11032, A-11034, A21245, Thermo Fisher Scientific).

    Techniques: Activity Assay, Western Blot, MANN-WHITNEY, Fluorescence, Staining, Confocal Microscopy, Reverse Transcription Polymerase Chain Reaction

    Increased IGFBP3-induced senescence in cells. (A) . Senescence markers were analyzed in parental (WT), Tau-KO (KO) or 10 μM Tazemetostat-treated parental (EZH2i) cells. The percent of senescence-associated βGal positive cells (βGal) was determined by automated cell imaging and reported as fold of WT; mean ± SD of 7–14 fields, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). The nuclear P16 fluorescent intensity (P16 IF) was determined with a DAPI nuclear mask (ImageJ) by immune fluorescence staining and laser confocal microscopy and reported as fold of WT mean value of 1991–3,153 nuclei unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). Living cells labeled with the acidotrophic dye LysoTracker (DOs) were imaged on a laser confocal microscope and analyzed for the size (area of 643–3,429 DOs) and mean number per cell (13–18 fields) of LysoTracker-positive organelles (ImageJ). Values are reported as fold of WT mean value (area; violin plot, high smoothing with median and quartiles) or mean ± SD (number), non-parametric Krustal-Wallis and Dunn’s multiple comparison test. (B) . Matched protein amounts of SH-SY5Y cells transduced with mock (ctrl) or IGFBP3 shRNA (IGFBP3-KD) pseudo lentiviral particles were analyzed by western blot (biological triplicates on a single gel) with IGFBP3 or GAPDH primary antibodies and anti-mouse IgG IRDye 680RD or anti-rabbit IgG IRDye 800CW secondary antibodies. The IGFBP3 signals were normalized for GAPDH and reported as fold of WT; mean ± SD of 9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (C) . As in (A) for SH-SY5Y cells transduced with mock (ctrl) or IGFBP3 shRNA (IGFBP3-KD) pseudo lentiviral particles. βGal: mean ± SD of 6 fields. P16 IF: violin plot of 669–881 nuclei; DOs; area of 456–551 DOs (violin plot), mean number per cell ± SD of DOs (5 fields); unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Tau protein modulates an epigenetic mechanism of cellular senescence in human SH-SY5Y neuroblastoma cells

    doi: 10.3389/fcell.2023.1232963

    Figure Lengend Snippet: Increased IGFBP3-induced senescence in cells. (A) . Senescence markers were analyzed in parental (WT), Tau-KO (KO) or 10 μM Tazemetostat-treated parental (EZH2i) cells. The percent of senescence-associated βGal positive cells (βGal) was determined by automated cell imaging and reported as fold of WT; mean ± SD of 7–14 fields, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). The nuclear P16 fluorescent intensity (P16 IF) was determined with a DAPI nuclear mask (ImageJ) by immune fluorescence staining and laser confocal microscopy and reported as fold of WT mean value of 1991–3,153 nuclei unpaired Mann-Whitney test. Violin plot, high smoothing with median and quartiles, GraphPad Prism 10.0.1 (218). Living cells labeled with the acidotrophic dye LysoTracker (DOs) were imaged on a laser confocal microscope and analyzed for the size (area of 643–3,429 DOs) and mean number per cell (13–18 fields) of LysoTracker-positive organelles (ImageJ). Values are reported as fold of WT mean value (area; violin plot, high smoothing with median and quartiles) or mean ± SD (number), non-parametric Krustal-Wallis and Dunn’s multiple comparison test. (B) . Matched protein amounts of SH-SY5Y cells transduced with mock (ctrl) or IGFBP3 shRNA (IGFBP3-KD) pseudo lentiviral particles were analyzed by western blot (biological triplicates on a single gel) with IGFBP3 or GAPDH primary antibodies and anti-mouse IgG IRDye 680RD or anti-rabbit IgG IRDye 800CW secondary antibodies. The IGFBP3 signals were normalized for GAPDH and reported as fold of WT; mean ± SD of 9 biological replicates, unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218). (C) . As in (A) for SH-SY5Y cells transduced with mock (ctrl) or IGFBP3 shRNA (IGFBP3-KD) pseudo lentiviral particles. βGal: mean ± SD of 6 fields. P16 IF: violin plot of 669–881 nuclei; DOs; area of 456–551 DOs (violin plot), mean number per cell ± SD of DOs (5 fields); unpaired Mann-Whitney test, GraphPad Prism 10.0.1 (218).

    Article Snippet: Detection by fluorescent laser confocal microscopy (Nikon C2 microscope) was done with 2 μg/mL secondary antibodies anti-mouse IgG Alexa594, anti-rabbit IgG -Alexa 488 or anti-rabbit IgG-Alexa 647 (A-11032, A-11034, A21245, Thermo Fisher Scientific).

    Techniques: Imaging, MANN-WHITNEY, Fluorescence, Staining, Confocal Microscopy, Labeling, Microscopy, Comparison, Transduction, shRNA, Western Blot